@article {2112,
	title = {SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs.},
	journal = {Nat Protoc},
	volume = {3},
	year = {2008},
	month = {10/2008},
	pages = {1941-74},
	abstract = {<p><span style="font-family: arial, helvetica, clean, sans-serif; font-size: 13px; line-height: 17px;">This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.</span></p>},
	keywords = {Image Processing, Computer-Assisted, Microscopy, Electron, Models, Molecular, Molecular Structure, Software, User-Computer Interface},
	issn = {1750-2799},
	doi = {10.1038/nprot.2008.156},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/19180078},
	author = {Shaikh, Tanvir R and Gao, Haixiao and Baxter, Bill and Asturias, Francisco J and Boisset, Nicolas and Leith, ArDean and Frank, Joachim}
}
